radiopej
Enlightened
I am in for the Thrunite TN35 please.
Wow, it's epic. Thanks to MoldyOldy for the chance :)
This is a gel showing some DNA bands. Basically, you extract DNA from a sample (in this case, bacteria). Then you amplify a small sequence like a gene using some primers and enzymes. This takes that sequence and doubles it over and over again (probably 25-35 times in this one). To confirm that it amplified, you can add it with a loading dye to a gel and run a current through it. DNA is negatively charged, so it moved towards the positive end of the gel tank. Bigger fragments move slower, so the lower it is the smaller it is. That series of bands on the left is ladder/size standard with fragments of known sizes. You compare the individual fragment sizes against that ladder to know the size. After you've run the gel for a set time, you drop it in ethidium bromide for a few minutes to let the staining agent bind to the DNA. Then you irradiate it with UV light to make the DNA glow. We reverse the image (saves ink on printing and is easier to see), so what you're seeing as dark black bands are actually bright.
That's an old gel that I picked so I can't remember what's on it. Judging by the size, it's probably a 16S sequence - you can use other methods to sequence the nucleotides of that fragment and compare it to millions and millions of others to identify the bacterium you isolated.
Wow, it's epic. Thanks to MoldyOldy for the chance :)
This is a gel showing some DNA bands. Basically, you extract DNA from a sample (in this case, bacteria). Then you amplify a small sequence like a gene using some primers and enzymes. This takes that sequence and doubles it over and over again (probably 25-35 times in this one). To confirm that it amplified, you can add it with a loading dye to a gel and run a current through it. DNA is negatively charged, so it moved towards the positive end of the gel tank. Bigger fragments move slower, so the lower it is the smaller it is. That series of bands on the left is ladder/size standard with fragments of known sizes. You compare the individual fragment sizes against that ladder to know the size. After you've run the gel for a set time, you drop it in ethidium bromide for a few minutes to let the staining agent bind to the DNA. Then you irradiate it with UV light to make the DNA glow. We reverse the image (saves ink on printing and is easier to see), so what you're seeing as dark black bands are actually bright.
That's an old gel that I picked so I can't remember what's on it. Judging by the size, it's probably a 16S sequence - you can use other methods to sequence the nucleotides of that fragment and compare it to millions and millions of others to identify the bacterium you isolated.
